Testing Proteins with Biurets Reagent

Testing Proteins with Biurets Reagent

Introduction

Protein is a major and indispensable class of cellular macromolecule . There be some proteins that help stick out cells and others that ar enzymes which help increase the rate of cellular reactions. non only do proteins provide vital functions in alive organisms but they are also used to transport substances surrounded by membranes and help regulate activity of other proteins and DNA. They either feed as hormones or help defend against infectious microorganisms. These proteins brood of macromolecules which are polymers consisting of one or more chains that are un-branched from monomers that are called amino acids. This molecule consists of both an amino and a carboxyl stem. Proteins can contain from as few as three to fifty-thousand amino acid units and range from shape and form, to organism completely soluble or insoluble to water solutions. When a protein is formed it is linked together by a peptide sequester using covalent bonds to form between an amino group of one amino acid and a carboxyl group. likewise dipeptides are formed when the peptide bond joins the two amino acids. Futhermore protiens are also linked by linking three amino acids and this is called a polypeptide.




The purpose of this experiment was to determine protein concentration in antithetic kind of substances using Biurets Reagent and assess if peptide bonds were present.If Biurets Reagent is added to the substances then the substances will smorgasbord to the color of Biurets Reagent. When adding Biurets Reagent to the different substances it will result in a change of color of blue depending on how many peptide bonds exist. If none exist then there will be no change in the substance.

Materials

The materials that were used in this experiment were instruments such(prenominal) as: Beakers disposable pipettes, test tubes, test tube holders, Chinese markers. The substances that were used were: Distilled H2O, glucose, starch, egg whites, sports drink,...If you want to get a replete(p) essay, order it on our website: Ordercustompaper.com



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